missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Competent Cells
  5. 11503127

Invitrogen™ ElectroMAX™ DH10B Cells

Código de producto. 11503127
Cambiar vista
Click to view available options
Quantity:
5 x 100 μL
Tamaño de la unidad:
Pack of 5
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Código de producto. Quantity unitSize
11503127 5 x 100 μL Pack of 5
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Este artículo no se puede devolver. Vea la política de devoluciones
Código de producto. 11503127 Proveedor Invitrogen™ N.º de proveedor 18290015
  • ONLINE EXCLUSIVE PRICE
    459.00€
    426.65€ / Pack of 5
    Save 32.35€
    7% Off
Ver todas las promociones
 Restricted Item
In Stock
Añadir a la cesta
Edge
280.00 EUR valid until 2026-08-14
BEST PRICE on Promo! Use promo code "27945" to get your promotional price.
Añadir a la cesta
Este artículo no se puede devolver. Vea la política de devoluciones

Descripción

Includes

ElectroMAX DH10B Cells: 5 vials, 100μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 2 bottles, 6mL each

Requires

A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA. The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.

ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited. They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

ElectroMAX DH10B Cell features
• Φ80lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• mcrA genotypic marker and mcrBC, mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation that enables increased plasmid yield and quantity

Genotype
F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG
Get more detailed information about specific Genotypes and Genetic Markers of our E. coli Competent Cells.

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats.
ElectroMax DH10B T1R Cells (Cat. No. 12033015) and MegaX DH10B T1R Electrocomp Cells (Cat. No. C640003) offer the benefit of T1 and T5 phage resistance.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Información de órdenes

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Especificaciones

Product Type Electrocompetent Cells
Contains F' Episome No
Improves Plasmid Quality Yes (endA1)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
Content And Storage • ElectroMAX DH10B Cells (5 x 100 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. medium (2 x 6 mL)
Store S.O.C. Medium at 4°C or room temperature.
Antibiotic Resistance Bacterial Yes (Streptomycin)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Blue/White Screening Yes (lacZΔM15)
High-throughput Compatibility Low
Plasmid May be used for plasmids >20 kb
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) No
Species E. coli (K12)
Format Tube
Product Line ElectroMAX
Quantity 5 x 100 μL
Mostrar más Mostrar menos

For Research Use Only. Not for use in diagnostic procedures.

Título del producto
Seleccione un problema

Al hacer clic en Enviar, acepta que Fisher Scientific se ponga en contacto con usted en relación con los comentarios que ha proporcionado en este formulario. No compartiremos su información para ningún otro fin. Toda la información de contacto proporcionada se mantendrá de acuerdo con nuestra Política de Privacidad. Política de privacidad.