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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

Product Code. 10532893
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20 Reactions
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20 reactions
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10532893 20 Reactions 20 reactions
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Product Code. 10532893 Supplier Invitrogen™ Supplier No. K250020

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Description

Includes

TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.

Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform

  • Contains minimal MCS, so sequence more of insert and less of vector
  • Patented att sites, pCR™8 vector to create entry clone, which facilitates easy access via Gateway recombination cloning to variety of expression vectors without tedious sub-cloning, saving valuable research time
  • att sites for rapid recombination into a variety of Gateway destination vectors
  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Sequencing primer sites within 55 bp of insertion site
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoRI sites flanking the PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Cloning Method TOPO™ with Gateway™
Content And Storage The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
No. of Reactions 20 reactions
Product Line One Shot
Product Type TA Cloning Kit
Quantity 20 Reactions
Vector pCR

For Research Use Only. Not for use in diagnostic procedures.

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