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Invitrogen™ pBAD/His Kit
Express the protein of interest with an N-terminal tag in a tightly regulated fashion
Brand: Invitrogen™ V43001
Additional Details : Weight : 0.14000kg
Includes: 20μg of each pBAD/His A, B & C vector, 20μg of pBAD/His/lacZ, 1mL of 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- araBAD promoter for tightly regulated expression
- Translation initiation signals optimized for E. coli expression
- N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody
- N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody
- Enterokinase cleavage site for removing the N-terminal tag following purification
- Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| His Tag (6x) | |
| pBAD |
| Arabinose | |
| Bacterial Expression Vector | |
| The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. | |
| EK (Enterokinase) Recognition Site | |
| Restriction Enzyme/MCS |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- pBAD/His Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362-Take off contaminated clothing.
For Research Use Only. Not for use in diagnostic procedures.