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Invitrogen™ pBAD/Myc-His Kit
Expresses native proteins or fusion proteins with a C-terminal tag in a tightly regulated fashion
Brand: Invitrogen™ V44001
Additional Details : Weight : 0.14000kg
Includes: 20μg of pBAD/Myc-His A, B and C vector, 20μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs.
Description
- araBAD promoter for tightly regulated expression
- Translation initiation signals optimized for E. coli expression
- C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with Anti-His(C-term) Antibody
- C-terminal c-myc epitope for detection and analysis with Anti-myc Antibody
- Three vectors are provided (A, B, and C): Each has C-terminal tag in different reading frame relative to multiple cloning site to simplify in-frame cloning of gene
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| Ampicillin (AmpR) | |
| c-Myc Epitope Tag | |
| pBAD |
| Arabinose | |
| Bacterial Expression Vector | |
| The pBAD/Myc-His Kit includes 20 μg of pBAD/Myc-His A, B, & C vector, 20 μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored. | |
| EK (Enterokinase) Recognition Site | |
| Restriction Enzyme/MCS |
For Research Use Only. Not for use in diagnostic procedures.