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Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit
Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector
Brand: Invitrogen™ K480001
763.00 EUR valid until 2026-05-15
BEST PRICE on Promo! Use promo code "27974" to get your promotional price.
Alert:
To receive the discount customers must purchase 2 of the same product at list price in a single order to receive 30% discount. There is no limit to the multiples of 2 that customers can buy. Use promo code ”27974” to get your promotional price
Beschreibung
- No ligase, post-PCR procedures, or PCR primers containing specific sequences are required
- Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines
- pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression
- C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody
- C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody
- Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants
- Vector features strong CMV promoter for high-level, constitutive expression
- C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Bestellinformation
Shipping Condition: Dry Ice
Spécification
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| His Tag (6x), V5 Epitope Tag | |
| 20 Reactions | |
| TOPO, pcDNA |
| Transfection | |
| TOPO TA Expression Kit | |
| Box 1 (store at -20°C) • 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl) • 100 μl 10X PCR Buffer • 10 μl dNTP Mix (50 mM) • 50 μl Salt Solution • 1 ml Sterile Water • 10 μl Control PCR Template (50 ng/μl) • 10 μl Control PCR Primers (100 ng/μl each) • 20 μl T7 Sequencing Primer (100 ng/μl) • 20 μl BGH Reverse Sequencing Primer (100 ng/μl) • 10 μl Expression Control Plasmid (500 ng/μl) Box 2 (store at -80°C) • 21 × 50 μl TOP10 E. coli cells • 50 μl pUC19 Control DNA (10 pg/μl) • 6 ml S.O.C. Medium |
|
| TOPO-TA | |
| TOPO-TA Vectors, pcDNA | |
| Constitutive Expression |
Produkttitel
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For Research Use Only. Not for use in diagnostic procedures.