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Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit
Description
Includes
The PureLink Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 50 preps.
PureLink™ Quick Gel Extraction and PCR Purification Combo Kit offers the ability to perform both a gel extraction or a PCR purification a single kit.
The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes. It also provide all components to perform a rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fragments >100 bp in less than 10 minutes. Isolated DNA using the PureLink™ kit is free of proteins, dye, and agarose, and is ready-to-use for a variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling. The kit contains all the buffers from the PureLink™ Quick Gel extraction kit and the PCR binding buffer from PureLink™ PCR Purification kit with Invitrogen branded lidded column.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Linear DNA fragments from: Agarose Gels, PCR Purification, Restriction Enzyme Reactions, PCR Products |
| Elution Volume | 50 μL |
| Final Product Type | DNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Label or Dye | Ethidium Bromide, SYBR Safe |
| Quantity | 50 Preps |
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Frequently Asked Questions (FAQs)
Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.
Yes, you can purify ANY dsDNA sample with a size range of 100 bp to 12 kb from liquid samples with the PureLink Quick PCR Clean-Up Kit. Supercoiled DNA can be purified as well, but some nicking should be expected.
The PureLink kit can be used with both TAE and TBE gels, while the S.N.A.P. Gel Purification Kit is recommended for TAE gels only.
The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.
Safety and Handling
- PureLink Quick Gel Extraction and PCR Purification Combo Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 C
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: Thiocyanic acid, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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