Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A⁺-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

• Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
• Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
• Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription