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  7. Invitrogen™ SuperScript™ III Reverse Transcriptase 

Invitrogen™ SuperScript™ III Reverse Transcriptase

Product Code. p-4926558
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No. of Reactions:
10 Reactions
200 Reactions
50 Reactions
Unit Size:
10ku
2ku
Pack of 4
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Produktkode 10432122

missing translation for 'mfr': Invitrogen™ 18080093

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98.15 EUR valid until 2025-12-19
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Beskrivelse

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript III Reverse Transcriptase include:
• First-strand cDNA may be synthesized at temperatures up to 55°C
• cDNA can be generated from 100 bp to >12 kb
• Increased specificity
• High yields of cDNA and full-length products

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat. No. 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

Ordreinfo

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Tekniske data

Concentration 200 U/μL
Content And Storage

• SuperScript™ III Reverse Transcriptase, 10 μL (2,000 units at 200 U/μL)
• 5X First-strand buffer, 1 mL
• DTT, 500 μL (100 mM)

Store at –20°C.
Format Stand-alone Enzyme
GC-Rich PCR Performance High
Reaction Speed 30 to 50 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C
Quantity 2,000 Units
Reverse Transcriptase SuperScript III
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Final Product Type First-Strand cDNA
No. of Reactions 10 Reactions
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 12.3 kb
Starting Material RNA
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For Research Use Only. Not for use in diagnostic procedures.

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