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  6. Invitrogen™ SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO 

Invitrogen™ SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO

Product Code. 10207252
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Quantity:
500 μL
1 mL
20 x 50 μL
Unit Size:
100µL
1mL
500µL
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10207252 1 mL 1mL
10710004 500 μL 500µL
11540746 20 x 50 μL 100µL
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Product Code. 10207252 Supplier Invitrogen™ Supplier No. S7567

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Description

Extremely sensitive stain for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels

SYBR Green I offers greater sensitivity for dsDNA, making it especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR Green I stain is ideal for use with laser scanners.
Product use 1mL stains 100 minigels.

  • Sensitivity: at least four times greater than ethidium bromide for DNA in agarose gels
  • Ease of use: gels soaked in diluted stain can be visualized without desalting
  • Compatibility: with UV transilluminators, gel documentation systems, and laser scanners
  • Flexibility: for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection

Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • Supplied as a 10,000X concentrate in DMSO

Store at -20°C, protected from light in a dessicator.
Detection Location In-Gel Detection
Detection Method Fluorescence
Label or Dye SYBR Green I
Quantity 1 mL
Shipping Condition Room Temperature
Target Molecule DNA
Product Type Nucleic Acid Gel Stain

Frequently Asked Questions (FAQs)

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain was specifically developed as a safer alternative to ethidium bromide. SYBR Green I is an ultrasensitive stain for dsDNA, and SYBR Green II is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

How should I dispose of SYBR DNA Gel Stain?

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays
Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

What is the stability of SYBR Green I and SYBR Gold Nucleic Acid gel stains?

When stored frozen at -20 degrees C or lower, desiccated and protected from light, the SYBR Green I stain and SYBR Gold stain stock solutions in DMSO are stable for one year from date of shipment.
Working solutions prepared in the recommended manner can be stored in an airtight, light-protected (foil wrapped or dark) polypropylene container in the refrigerator between uses. Under these conditions, working solutions will reliably last 3 or 4 days.
Staining solutions prepared in buffers with pH below about 7.0 or above 8.5 are less stable and show reduced staining efficacy.
Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity.

We recommend storing aqueous working solutions in plastic rather than glass, as the stain may adsorb to glass surfaces.

For Research Use Only. Not for use in diagnostic procedures.

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