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Description
Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.
SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.
Highlights:
- Efficient – 100% label incorporation into proteins of living cells
- Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases
- Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment
- High-quality supplements – heavy amino acids with >99% isotope purity
Spécification
Spécification
| For Use With (Equipment) | Mass Spectrometer |
| Product Type | Amino Acids |
| Content And Storage | Store at room temperature. |
| Label or Dye | Mass Isotope of Amino Acid |
| Shipping Condition | Room Temperature |
| Starting Material | Cells |
| Final Product Type | Protein |
| Detection Method | Mass Spectrometry |
| Workflow Step | Protein Labeling |
| Quantity | 50 mg |
| Afficher plus |
For Research Use Only. Not for use in diagnostic procedures.
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