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Description
- Lentil lectin coupled to Sepharose 4B via cyanogen bromide activation
- Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
- Retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteins
- Ligand concentration 2 mg Lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16mg/mL medium
- Average particle size 90μm
- Stable in the pH range 3-10, and in the presence of all commonly used buffers (Chelating agents and 8M urea to be avoided)
- Has more equal affinity for α-D-mannose and α-D-glucose compared to Con A
Order Info
Supplied in 20% ethanol containing 0.9% NaCl, 1mM Ca2+ and 1mM Mn2+
Specifications
Specifications
| Binding Capacity | 25mg/mL |
| Ligand Concentration | 2mg Lentil lectin/mL Medium |
| Matrix | 4% Agarose |
| Particle Size | 90 μm |
| pH | 3 to 10 |
| Quantity | 25 mL |
| Content And Storage | 2°C to 8°C |
| For Use With (Application) | Used for purification of a number of Glycoproteins and other Carbohydrate containing molecules |
| Ligand | Lentil lectin |
Safety and Handling
Product Identifier
- lentil lectin sepharose 4B
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol
Product Title
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