missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Description
The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH. APC/Cy5.5 is a tandem conjugate. In the Lightning-Link APC/Cy5.5 the tandem is excited at 633nm, wavelength at which the APC has its maximum absorption. The excited APC functions then as an energy donor, where the energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5.5. The Cy5.5 emits then the energy received from the APC in the form of long wavelength light at 694nm. The amount of antibody used for labeling should be the same as the pack size of LL-APC-Cy5.5. For any new antibody, optimization of the ratio of antibody to dye is often worthwhile. If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.
Order Info
Quantity
Specifications
Specifications
| Content And Storage | Store at -20°C. Avoid freeze-thaw cycles. |
| Quantity | 100 μg |
| Conjugate | APC/Cy5.5 |
| Kit Contents | Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent |
| Product Type | APC-Cy5.5 Antibody Labeling Kit |
Product Title
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.
Spot an opportunity for improvement?