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Thermo Scientific™ LightShift™ Chemiluminescent RNA EMSA Kit

Código de producto. 10780910 Tienda Thermo Scientific Productos
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Quantity:
100 reactions
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Descripción

Study RNA-protein interactions with this non-radioactive EMSA kit for gel shift assays.

Thermo Scientific™ LightShift Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

The RNA EMSA Kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods. The complete kit comes with all reagents required to set up and optimize protein-RNA binding conditions, a positive control for protein-RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction.

Highlights:

  • Sensitive – chemiluminescent detection comparable to radioactive detection

  • Time-saving – develop X-ray films after 1- to 5-minute exposures, versus 16 hour exposures needed with radioactive systems

  • Flexible – compatible with RNA probes biotinylated by different methods

  • Easy to use – complete kit includes optimized reagents for binding reactions and RNA probe detection

  • Non-radioactive – eliminate waste concerns from radioactive RNA probes

TRUSTED_SUSTAINABILITY

Especificaciones

Assay EMSA Assay
Content And Storage Sufficient For: 100 binding reactions and detection on 1000 cm2 of membrane (10 mini-gel blots)• 125 nM Biotinylated IRE RNA Control 35 μL (store at -20°C)
• 10 μM Unlabeled IRE RNA Control, 25 μL (store at -20°C)
• 2 mg/mL Cytosolic Liver Extract, 50 μL (store at -20°C)
• 10 mg/mL tRNA, 100 μL (store at -20°C)
• 10X REMSA Binding Buffer, 1 mL (store at -20°C)
• 50% Glycerol, 500 μL (store at -20°C)
• 2M KCl, 500 μL (store at -20°C)
• 1M MgCl2, 500 μL (store at -20°C)
• DTT, lyophilized (store at -20°C)
• Nuclease-Free Water, 1.5 mL (store at -20°C)
• 5X REMSA Loading Buffer, 1 mL (store at -20°C)
• Stabilized Streptavidin-HRP, 1.5 mL (store at 4°C)
• Luminol/Enhancer Solution, 80 mL (store at 4°C)
• Stable Peroxide Solution, 80 mL (store at 4°C)
• Nucleic Acid Detection Blocking Buffer, 500 mL (store at 4°C)
• 4X Wash Buffer, 500 mL (store at 4°C)
• Substrate Equilibration Buffer, 500 mL (store at 4°C)
Detection Method Chemiluminescence
Format Kit
Sample Type Protein
For Use With (Equipment) myECL™ Imager, X-Ray Film
No. of Reactions 100 reactions
Product Line LightShift
Product Type LightShift Chemiluminescent RNA EMSA Kit
Quantity 100 reactions
Target Specificity Not Target-Specific
Technique Enhanced Chemiluminescence, Membrane Blot, Gel Shift
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For Research Use Only. Not for use in diagnostic procedures.

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