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  6. Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Product Code. 30245142
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Quantity:
100 Reactions
120 μg
1600 μg
2000 μL
Product Type:
Lyo-ready RPA Kit
Lyo-ready T4 Gene 32 Protein
Lyo-ready T4 UvsX Protein
Lyo-ready T4 UvsY Protein
Reaction Buffer
Unit Size:
100 reactions
120µg
1600µg
2000µL
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Product Type unitSize
30245142 100 Reactions Lyo-ready RPA Kit 100 reactions
30245335 1600 μg Lyo-ready T4 Gene 32 Protein 1600µg
30245214 120 μg Lyo-ready T4 UvsX Protein 120µg
30245152 120 μg Lyo-ready T4 UvsY Protein 120µg
30245027 2000 μL Reaction Buffer 2000µL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
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This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Product Code. 30245142 Supplier Invitrogen™ Supplier No. A72127

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177.34 EUR valid until 2026-03-20
BEST PRICE on Promo! Use promo code "27968" to get your promotional price.
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Description

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY

Specifications

Product Type Lyo-ready RPA Kit
Form Liquid
For Use With (Application) RPA, RT-RPA
Polymerase Lyo-ready Bst DNA Polymerase
Quantity 100 Reactions
Research Category Nucleic Acid Amplification
Content And Storage

• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL

Store at -25°C to -15°C.
Optimal Reaction Temperature 42°C
Reverse Transcriptase SuperScript IV Reverse Transcriptase
Shipping Condition Dry Ice
Detection Method End point, real-time, lateral flow
Reaction Time Up to 20 min.
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For Research Use Only. Not for use in diagnostic procedures.

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