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Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
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Includes
First Strand Enzyme Mix, 4X First Strand Reaction Mix, Second Strand Enzyme Mix, 5X Second Strand Reaction Mix, 0.5M EDTA ( pH 8.0), RNase I (10 u/μL), Control RNA (0.5μg/μL), Oligo(dT)18 Primer, 100μM, 0.5μg/μL, Random Hexamer Primer, 100μM, 0.2μg/μL,Water, nuclease-free, Detailed Protocol
Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.
Features of the Maxima H Minus Double-Stranded cDNA Synthesis Kit include:
- Efficient synthesis of full-length double-stranded cDNA
- Fast—procedure completed in less than two hours
- Convenient—pre-mixed components
- Complete—includes all primers, controls and residual RNA removal reagents
Applications
- Full-length double-stranded blunt-end cDNA synthesis for cloning
- Double-stranded cDNA library construction
Order Info
Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.
Specifications
Specifications
| Format | 10 reactions |
| Optimal Reaction Temperature | 50°-55° C |
| Product Line | Maxima |
| Product Type | cDNA Synthesis Kit |
| Quantity | 10 Reactions |
| Reverse Transcriptase | Maxima H Minus |
| For Use With (Application) | Cloning |
| Final Product Type | Double Stranded cDNA |
| No. of Reactions | 10 Reactions |
| Reaction Format | Separate Components |
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Safety and Handling
- Maxima H Minus Double-Stranded cDNA Synthesis Kit
- Warning
- Specific target organ toxicity after repeated exposure Category 2
- H373-May cause damage to organs.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P314-Get medical advice/attention if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : EDTA
For Research Use Only. Not for use in diagnostic procedures.
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