Gibco™ Medium 199, Hanks' Balanced Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. This Medium 199 formulation contains Hanks' salts for use without CO₂.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

Dual-Site cGMP Manufacturing
Gibco™ Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ Medium 199 product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Medium 199 formulation does not require a CO₂ environment to maintain physiological pH.