Gibco™ MEM α, nucleosides, powder

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red

Without: Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.