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Invitrogen™ Carboxy-H2DCFDA (general oxidative stress indicator)

Product Code. 11500146
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25 mg
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25mg
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11500146 25 mg 25mg
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Product Code. 11500146 Supplier Invitrogen™ Supplier No. C400
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Description

General oxidative stress indicator

6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. This nonfluorescent molecule is readily converted to a green-fluorescent form when the acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within the cell. We offer the carboxylated H2DCFDA analog (carboxy-H2DCFDA, Cat. No. C400), which has two negative charges at physiological pH, and its di(acetoxymethyl ester) (Cat. No. C2938). Upon cleavage of the acetate and ester groups by intracellular esterases and oxidation, both analogs form carboxydichlorofluorescein (Cat. No. C368), with additional negative charges that impede its leakage out of the cell.

ROS Indicator Specifications:

  • Ex/Em: ∼492–495/517–527 nm
  • Product is air sensitive and should be stored under dry argon or nitrogen
  • Product may be dissolved in DMSO, DMF, or ethanol for use
  • Indicator is cell permeant (cell loading protocols are available in the literature)
  • Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein

Find More ROS Indicators

We offer an assortment of Molecular Probes™ products for the generation of reactive oxygen species (ROS), including singlet oxygen, superoxide, hydroxyl radical and various peroxide and hydroperoxides, as well as for their fluorometric detection in solution. Review Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

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Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Quantity 25 mg
For Use With (Application) ROS
Detection Method Fluorescence
Product Type ROS Indicator
Excitation Wavelength Range 492 to 495 nm
Emission Wavelength Range 517 to 527 nm

For Research Use Only. Not for use in diagnostic procedures.

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