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Invitrogen™ CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

Product Code. 13599180
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1.75 mL kit
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13599180 1.75 mL kit 1.75mL
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Product Code. 13599180 Supplier Invitrogen™ Supplier No. R37111

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317.64 EUR valid until 2026-04-30
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Description

Includes

1 x 1.75mL dropper bottles

Fluorogenic, no-wash indicator for live- and fixed-cell applications

Activation of caspase-3 is an early indicator of apoptosis.

  • Allows rapid and sensitive detection of cells destined for cell death
  • Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
  • Optimized caspase-3/7 substrate for apoptosis analysis
  • Simple and fast no-wash protocol helps preserve delicate apoptotic cells
  • Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods
  • Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
  • Four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is nonfluorescent when not bound to DNA: The Caspase-3/7 Green reagent is intrinsically nonfluorescent, as DEVD peptide inhibits binding of dye to DNA
  • Upon activation of caspase-3/7 in apoptotic cells, DEVD peptide is cleaved and free dye can bind DNA, generating bright green fluorescence
  • Apoptotic cells with activated caspase-3/7 will have bright green nuclei, while cells without activated caspase 3/7 will show minimal fluorescence
  • Fluorescent signal survives fixation and permeabilization, allowing end-point assays and immunocytochemistry-based methods
  • Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until optimal staining intensity is obtained
  • If desired, combine with red or far-red nuclear stain for total cell count
  • Excited with maximum at 502nm and has emission maximum at 530nm
  • It is detected through standard GFP and FITC filters

Apoptosis, Apoptosis Related Factors, Caspase Activation, Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, High-Content Screening (HCS)

TRUSTED_SUSTAINABILITY

Specifications

Quantity 1.75 mL kit
Format Dropper Bottle
Product Type Caspase 3/7 Reagent
Content And Storage 1 × 1.75 mL dropper bottles

Store at ≤ 25°C.
Compatible Cells Mammalian Cells
Excitation/Emission 502/530
Color Green
Product Line CellEvent, ReadyProbes
Detection Method Fluorescence
For Use With (Application) Cell Imaging
For Use With (Equipment) Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Flow Cytometer, Floid™ Cell Imaging System
Label Type Other Label(s) or Dye(s)
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Frequently Asked Questions (FAQs)

I want to use your CellEvent Caspase-3/7 Green detection reagent for studying apoptosis in live cells, but for 96-well microplate instead of microscopy. Will it work? Can I multiplex it with a cell vitality dye?

Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.
I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.
What are the recommended storage conditions for the CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111)?

The recommended storage conditions for CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111) is between 4 and 25 degrees C or room temperature.

How long can I measure the Cell Event Caspase-3/7 Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) signal for the kinetic assay?

We have tested Caspase 3/7 Green Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) for up to 48 hours and observed no toxicity or stability issues with the reagent. If the samples are fixed at the end of the time course the signal will be retained for days. For live cells, apoptotic cells will round up and float away before the loss of fluorescence signal from CellEvent.

The following reference reports a visible signal for up to 72 hrs:

Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020 May 25;14:1995-2019. doi: 10.2147/DDDT.S242514. PMID: 32546966; PMCID: PMC7260544.
Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.
Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.
With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.
With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.
Can I fix my CellEvent Caspase 3/7 - labeled cells?

For imaging applications, we recommend fixing with 3.7% formaldehyde at room temperature for 15 mins. After fixation, cells can be imaged for up to 24 hrs, but the signal will gradually degrade over time. For flow cytometry applications, fixation is not recommended.
I wish to measure cell proliferation in combination with apoptosis in a microplate assay. Do you have any product recommendations?

It is possible to combine PrestoBlue reagent (for cell proliferation) with the CellEvent Caspase 3/7 Green Detection Reagent (for apoptosis detection) in the same sample.
What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.
What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

For Research Use Only. Not for use in diagnostic procedures.

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