Invitrogen™ Click-IT™ Biotin Protein Analysis Detection Kit

The Click-iT Biotin Glycoprotein Detection Kit provides the second part of a simple and robust technique to identify and characterize glycoproteins by Western blot. In step two, after the incorporation of an azide moiety into protein glycan structures with either a Click-iT metabolic labeling reagent or the Click-iT Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. With this technique, detection sensitivity is in the low femptomole range and biotin-labeled samples can be detected before or after probing the Western with a primary antibody.

• Less hazardous

Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis, Western Blotting