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Invitrogen™ Vybrant™ Phagocytosis Assay Kit

Product Code. 10552903
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Product Code. 10552903 Supplier Invitrogen™ Supplier No. V6694

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Description

Observe and quantitate process of phagocytosis in human polynuclear cells and mouse macrophages by following internalization of a foreign particle

  • Foreign particle is killed, E. coli (K-12 strain) cells that have been labeled with fluorescent dye fluorescein
  • Addition to lyophilized E. coli BioParticles™ component, kit contains a trypan blue solution (to quench fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader
  • Methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines
  • Particles are fluorescein-labeled E. coli (K-12 strain)
  • Kit supplies sufficient reagents for approx. 250 tests using 96-well plates
  • Fluorescence is measured using a microplate reader (Ex/Em approx. 480/520nm)

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Product Type Phagocytosis Assay Kit
Dye Type Classic Dyes
Format 96-well plate
Product Line Vybrant
Quantity 1 kit
Shipping Condition Room Temperature
Species E. coli

Frequently Asked Questions (FAQs)

What is phagocytosis?

Phagocytosis is an important mechanism for nourishment in unicellular organisms and for defense against infection in higher vertebrates. The process of phagocytosis can be observed and quantitated in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle such as fluorescently labeled immune complexes and bacterial particles.

Which medium should I use for culturing cells to be used with the Vybrant Phagocytosis Assay Kit (Cat. No. V6694)?

We recommend using any medium that is optimal for culturing your cell(s) of interest. Avoid media that have extremes of pH, heavy metals or strong oxidizing/reducing agents as these may degrade the dye fluorescein on the BioParticles and may degrade or precipitate Trypan blue dye.

For Research Use Only. Not for use in diagnostic procedures.

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