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Invitrogen™ Vybrant™ Phagocytosis Assay Kit
Description
- Foreign particle is killed, E. coli (K-12 strain) cells that have been labeled with fluorescent dye fluorescein
- Addition to lyophilized E. coli BioParticles™ component, kit contains a trypan blue solution (to quench fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader
- Methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines
- Particles are fluorescein-labeled E. coli (K-12 strain)
- Kit supplies sufficient reagents for approx. 250 tests using 96-well plates
- Fluorescence is measured using a microplate reader (Ex/Em approx. 480/520nm)
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Microplate Reader |
| Product Type | Phagocytosis Assay Kit |
| Dye Type | Classic Dyes |
| Format | 96-well plate |
| Product Line | Vybrant |
| Quantity | 1 kit |
| Shipping Condition | Room Temperature |
| Species | E. coli |
For Research Use Only. Not for use in diagnostic procedures.
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