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Proteintech™ Mouse Leptin ELISA Kit

Product Code. 16891636 Shop All Proteintech Products
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Target Species:
Mouse
Unit Size:
96 tests
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Product Code. 16891636

Brand: Proteintech™ KE1004896T

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This item is not returnable. View return policy

Description

KE10048 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse Leptin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Leptin.

  • The assay recognizes mouse Leptin. An antibody specific for mouse Leptin has been pre-coated onto the microwells.
  • The mouse Leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Leptin is added to detect the captured mouse Leptin protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
  • Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance.
  • Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions.
  • Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

Specifications

Accession Number BC038162
Assay Range 62.5 to 4000 pg/mL
Assay Sensitivity 0.4 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Mouse
Gene ID (Entrez) 16846
Host Species Mouse
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P41160
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Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • 96TEST Mouse Leptin ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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