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  5. Thermo Scientific™ Nitrocellulose Membranes, 0.45 μm

Thermo Scientific™ Nitrocellulose Membranes, 0.45 μm

Product Code. 10270584 Shop All Thermo Scientific Products
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Dimensions (LxW):
10.5 x 7.9 cm
12 x 8 cm
3.5 m x 30 cm
8 x 8 cm
Format:
Roll
Sheet
Sufficient For:
≥120 Mini-gel Blots (When cut to 8 x 10 cm)
15 Mini-gel Blots
25 Mini-gel Blots
Unit Size:
Each
Pack of 15
Pack of 25
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Product Code. Dimensions (LxW) Format Sufficient For Unit Size
10270584 8 x 8 cm Sheet 15 Mini-gel Blots Pack of 15
10551584 10.5 x 7.9 cm Sheet 15 Mini-gel Blots Pack of 15
10168543 12 x 8 cm Sheet 25 Mini-gel Blots Pack of 25
10773485 3.5 m x 30 cm Roll ≥120 Mini-gel Blots (When cut to 8 x 10 cm) Each
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This item is not returnable. View return policy
Product Code. 10270584 Supplier Thermo Scientific™ Supplier No. 88025
Protein Biology Essentials
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Description

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).

Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods). The 0.45-μm pore size is well-suited for a wide range of western blotting conditions and ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

Features include:
• 100% pure nitrocellulose membrane
• Convenient—save time with pre-cut sheets or choose the roll format for the flexibility to cut to the ideal size
• Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• High-binding affinity—provides excellent protein binding, blocks easily, and does not require pre-wetting with alcohol
• Low background: produce high-quality data with low background signal in both chemiluminescent and fluorescent western blotting

TRUSTED_SUSTAINABILITY

Specifications

Length (Metric) 8 cm
Width (Metric) 8 cm
Description Nitrocellulose Membranes, 0.45 μm, 8 x 8 cm
Binding Capacity ∼45–60 μg/cm2 of BSA
Quantity 15 Pre-cut Membranes
Format Sheet
Material Nitrocellulose
Pore Size 0.45 μm
Content And Storage Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry and become brittle.
Note: Nitrocellulose membrane filters are highly flammable. Keep away from heat and open flame. Flash point is approximately 2°C. Membranes can be sterilized by steam-autoclaving at 121°C.
Shipping Condition Room Temperature
Dimensions (LxW) 8 x 8 cm
Sufficient For 15 Mini-gel Blots
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Seguridad y manipulación

compliance-icons
Nombre del producto
  • NITROCELLULOSE MEMBRANE, 0.45U
 Palabra de advertencia
  • Danger
 Clasificación
  • Flammable solid Category 1
 Indicaciones de peligro
  • H228-Flammable solid.
 Consejos de prudencia
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P240-Ground/bond container and receiving equipment.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
 Otros peligros
  • MIXTURE LIST-Contain: Nitrocellulose
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