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Macherey-Nagel™ NucleoSpin™ 96 PCR Clean-up, 96-well kit for PCR clean up
Descripción
The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.
- Silica membrane technology
- Processing: manual/automated, vacuum/centrifugation
- Sample material: < 10 0μL PCR reaction mixture
- Elution volume: 75 to 150 μL
- Binding capacity: 15 μg
- Preparation time: 45 min./plate
- Fragment size: 65 bp to 10 kb
Especificaciones
Especificaciones
| Content And Storage | 18°C to 25°C |
| Format | 96-well Plate |
| For Use With (Application) | PCR clean up |
| No. of Reactions | 384 Preps |
| Sample Volume | <100 μL |
Seguridad y manipulación
- Warning
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
- MIXTURE LIST-Contains : guanidinium thiocyanate
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