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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Product Code. 11992462
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No. of Reactions:
10 Preps
240 Preps
50 Preps
Förpackningsstorlek
10 reactions
240 preps
50 reactions
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Produktkode 11992462

missing translation for 'mfr': Macherey-Nagel™ 740609.10

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Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Tekniske data

Color pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Efficiency Typical recovery: 70–95%
Elution Volume Elution volume: 15–30μL
For Use With (Equipment) TAE or TBE agarose gels, PCR
Includes Binding Buffer NTI (10mL), Wash Buffer NT3 Concentrate (6mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (10), 2mL Collection Tubes (10)
No. of Reactions 10 Preps
Quantity 1 Kit
Purity A260/A280: 1.8–1.9
Sample Volume <200 μL PCR reaction mixture, <200 mg agarose gel
Size 50 bp to ∼20 kbp
Test Time 10 min/6 preps
Yield Up to ∼15μg DNA
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Signalord
  • Warning
Farekategori
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
Faresætning
  • H302-Harmful if swallowed.
  • H412-Harmful to aquatic life with long lasting effects.
Supplerende oplysninger
  • MIXTURE LIST-Contains : guanidinium thiocyanate
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