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  5. Macherey-Nagel™ NucleoSpin™ Plasmid DNA

Macherey-Nagel™ NucleoSpin™ Plasmid DNA

Product Code. 12353358
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No. of Reactions:
10 Preps
12 Preps
250 Preps
50 Preps
Includes:
Lid
No Lid
Unit Size:
10 reactions
12 preps
250 reactions
50 reactions
Product Code. No. of Reactions Includes unitSize
12353358 250 Preps Lid 250 reactions
11942502 10 Preps Lid 10 reactions
11932392 50 Preps Lid 50 reactions
11912252 250 Preps No Lid 250 reactions
15186446 12 Preps No Lid 12 preps
11942362 50 Preps No Lid 50 reactions
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This item is not returnable. View return policy

Product Code. 12353358

Brand: Macherey-Nagel™ 740588.250

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This item is not returnable. View return policy

Description

Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid Columns come with or without a cap lid.

Features:

  • For rapid small scale preparation high-purity plasmid DNA
  • Fast procedure – 6 Minipreps in 25 min.
  • High capacity – up to 50μg of plasmid DNA
  • Chaotropic salt wash buffer – extra pure DNA

Applications:

  • High-copy plasmids from E. coli
  • Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
  • Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
  • Supplementary protocol for M13 DNA available on request
  • Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

Specifications

Format Column
Isolation Technology Silica Membrane Technology
Sequencing Type DNA and RNA Sequencing
For Use With (Application) High-copy plasmids from E. coli
Includes Lid
No. of Reactions 250 Preps
Product Type Plasmid Columns
Quantity 2 mL
Sample Volume 2 to 10 mL
Size <50 kbp
Test Time 25 min.
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt/guanidine hydrochloride/2-pr
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