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  5. Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification

Macherey-Nagel™ NucleoSpin™ RNA Columns, Mini columns for RNA purification

Product Code. 15870968
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250 Columns
50 Columns
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Pack of 250
Pack of 50
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Product Code. Quantity unitSize
15870968 250 Columns Pack of 250
15880968 50 Columns Pack of 50
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Product Code. 15870968 Supplier Macherey-Nagel™ Supplier No. 740955.250S

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Description

Additional silica-membrane mini spin columns for use in isolating RNA of the highest integrity from cells and tissue using the NucleoSpin™ RNA kit.

NucleoSpin™ RNA

  • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
  • NucleoSpin Filters (shredders) – efficient sample homogenization and reduction of viscosity
  • Up to 70 μg ready to use RNA
  • Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
  • RNA isolation from cultured cells, tissue (standard protocol):
    • RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
    • RNA from ≤ 100 μL biological fluids
    • RNA clean-up from reaction mixtures
    • RNA from samples stored in RNAlater™
    • RNA from saliva samples collected with Oragene™ RNA (Genotek)
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

Color Blue band
Content And Storage rDNase at 4 °C; Other components at room temperature (18°C to 25 °C)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Elution Volume 40 to 120 μL
Sample Type Cultured Bacterial or Yeast Cells, Human or Animal Tissue
Efficiency Binding capacity: 200 μg
For Use With (Equipment) NucleoSpin RNA Kits
For Use With (Application) Isolation of RNA from cells and tissue
Includes Columns only
Quantity 250 Columns
Sample Size < 30 mg tissue, 5 x 106 cultured cells
Test Time 35 min/6 preps
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Safety and Handling

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Product Identifier
  • NucleoSpin RNA
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 B
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); gu
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