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Macherey-Nagel™ NucleoSpin™ RNA Plus

Product Code. 15300205
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250 Preps
This item is not returnable. View return policy

Product Code. 15300205

Brand: Macherey-Nagel™ 740984.250

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This item is not returnable. View return policy

Description

State-of-the-art, dual-column kit includes gDNA removal columns and RNA binding columns for efficient and quick RNA isolation from cells and tissues.

NucleoSpin™ RNA Plus

  • Efficient and quick RNA isolation from cells and tissues
  • Two-column system includes gDNA removal columns and RNA binding columns
  • NucleoSpin™ gDNA Removal Columns – no time consuming rDNase digestion
  • Convenient handling – lysate clearing and gDNA removal on one column in one step
  • New efficient lysis buffer – no b-mercaptoethanol or TCEP necessary
  • Variety of sample materials – cultured cells, animal tissue, plant tissue, microorganisms
  • Downstream applications include real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Specifications

Color Yellow/Blue Ring
Content And Storage Room temperature (18°C to 25°C)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Elution Volume 30 to 120 μL
Sample Type Cultured Bacterial or Yeast Cells, Human or Animal Tissue
Efficiency Binding capacity: 200 μg
Final Product Type RNA (with typical RIN > 9)
For Use With (Equipment) Centrifuge
For Use With (Application) Isolation of RNA
Includes NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers
No. of Reactions 250 Preps
Product Type RNA Isolation Kit
Purity A260/A280: 1.9 - 2.1
Sample Size < 30 mg tissue; < 30 mg tissue
Test Time 20 min./6 preps
Yield HeLa cells (5 x 106): 40-60 μg
Mouse liver (20 mg): 80-100 μg
Mouse kidney (20 mg): 40-70 μg
Mouse spleen (5 mg): 30-60 μg
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