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  5. 11964832

OPTIZYME™ XmaJI (AvrII), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8082-1

Additional Details : Weight : 0.01000kg

Product Code. 11964832

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...C^C T A G G...3'
3'...G G A T C^C...5'

Supplied with: 10X OPTIZYME 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 50 -100%
  • Buffer 3: 50 - 100%
  • Buffer 4: 100%
  • Buffer 5: 50 - 100%

Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with XmaJI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 10 units of XmaJI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: BcuI, NheI, XbaI, Eco130I (C^CTAGG)
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50 fold over-digestion with XmaJI
    200 U
    XmaJI (AvrII)
    10X OPTIZYME™ Buffer 4
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    C.CTAGG
    Liquid
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