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Thermo Scientific™ PCR Master Mix (2X)
Optimize routin PCR with this highly thermostable DNA polymerase.
Brand: Thermo Scientific™ K0171
Additional Details : Weight : 1.51950kg
Description
A 950 bp single copy gene from human genomic DNA was amplified at a wide range of Mg2+ concentrations.
M - GeneRuler 100 bp Plus DNA Ladder
Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.
PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Thermostable -half life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Supplied with two buffers -10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Specifications
| Contains: • 4 x 1.25 mL PCR Master Mix (2X), which includes: — Taq DNA polymerase (0.05 U/μL), — reaction buffer, — 4 mM MgCl2, and — 0.4 mM of each dNTP • 4 x 1.25 mL Nuclease-free water Store at -20°C. |
|
| Taq DNA Polymerase | |
| PCR Master Mix (2X) | |
| 1 X | |
| 3'-A |
| Low | |
| Standard | |
| 200 Reactions | |
| No | |
| SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.