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Thermo Scientific™ PCR Master Mix (2X)
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Description
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
Specifications
Specifications
| Concentration | 2X |
| Content And Storage | • 2X PCR Master Mix, 4 x 1.25 mL • Nuclease-free water, 4 x 1.25 mL Store at -20°C. |
| GC-Rich PCR Performance | Low |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix |
| Quantity | 200 reactions |
| For Use With (Application) | Standard PCR |
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
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For Research Use Only. Not for use in diagnostic procedures.
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