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  4. Gibco™ PeproGMP™ Human TPO (Thrombopoietin) Recombinant Protein, PeproTech®

Gibco™ PeproGMP™ Human TPO (Thrombopoietin) Recombinant Protein, PeproTech®

Product Code. 30275503
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Quantity:
100 μg
50 μg
500 μg
Unit Size:
100µg
50µg
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Product Code. 30275503

Brand: Gibco™ GMP30018100UG

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Description

Recombinant Protein

Thrombopoietin (TPO) is a hematopoietic growth factor that stimulates the proliferation and maturation of megakaryocytes. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. PeproTech Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. PeproGMP TPO cytokine is most often used with PeproGMP SCF and PeproGMP Flt3-ligand in ex-vivo cell therapy for the proliferation of hematopoietic stem cell (HSC) cultures, along with IL-3 and IL-6 for further synergistic expansion and immune cell maturation. Reconsitute using sterile water (i.e., WFI) to a concentration of 0.5-1.0 mg/ml. Additional product information , specifications, handling and reconstitution information are provided on the Certificate of Analysis. All PeproGMP™ products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P40225
For Use With (Application) Control, Western Blot Control, Bioactivity
Format Lyophilized
Formulation Protein with no preservative
Gene ID (Entrez) 7066
Molecular Weight (g/mol) 18.6 kDa
Name PeproGMP™ Human TPO (Thrombopoietin)
Purification Method Purified
Quantity 100 μg
Storage Requirements -20°C or -80°C if preferred
Regulatory Status GMP
Endotoxin Concentration ≤0.1 EU/μg
Gene Alias C-mpl ligand; Megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; Mgdf; MKCSF; ML; MPL ligand; Mpllg; MSA; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; TDH2A; THCYT1; THPO; Thrombopoietin; thyroid microsomal antigen; thyroid peroxidase; thyroperoxidase; Tpo; TPX
Common Name Thrombopoietin
Gene Symbol THPO
Biological Activity Determined by its ability to stimulate human MO7e cell proliferation. PeproGMP Recombinant HumanTPO has been tested against the WHO standard NIBSC 03/124.
Product Type Protein
Conjugate Unconjugated
Recombinant Recombinant
Sequence SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Content And Storage -20°Cor to +80°C if preferred
Activity Determined by its ability to stimulate human MO7e cell proliferation. PeproGMP Recombinant HumanTPO has been tested against the WHO standard NIBSC 03/124. Find the International Unit (IU) value <a href='https://www.thermofisher.com/us/en/home/life-science/cell-culture/cell-culture-learning-center/recombinant-protein-information/international-units.html'>here</a>.
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 98% by SDS-PAGE gel and HPLC analyses.
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