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Thermo Scientific™ Phusion™ HF and GC Buffer Packs

Product Code. 10492088 Shop All Thermo Scientific Products
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Chemical Name or Material:
Detergent-free FF Buffer Pack
Detergent-free GC Buffer Pack
GC Buffer Pack
HF Buffer Pack
Color:
Colorless
Green
Unit Size:
Pack of 4
6 product options available for selection
Product selection table with 6 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Chemical Name or Material Color unitSize
10492088 HF Buffer Pack Colorless Pack of 4
10584578 GC Buffer Pack Colorless Pack of 4
10226009 Detergent-free FF Buffer Pack Colorless Pack of 4
10333609 Detergent-free GC Buffer Pack Colorless Pack of 4
13525170 HF Buffer Pack Green Pack of 4
13535170 GC Buffer Pack Green Pack of 4
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 6 options available.
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Product Code. 10492088 Supplier Thermo Scientific™ Supplier No. F518L

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This item is not returnable. View return policy

Description

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material HF Buffer Pack
Color Colorless
Description Phusion™ HF Buffer Pack
For Use With (Application) Standard PCR
Quantity 4 x 1.5 mL
Includes • 5X Phusion HF Reaction Buffer (4 x 1.5 mL)
• 50 mM MgCl2 (4 x 1.5 mL)
• DMSO (0.5 mL)

Store at –20°C.

For Research Use Only. Not for use in diagnostic procedures.

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