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Description
Thermo Scientific™ Pierce DSG is disuccinimidyl glutarate, a simple homobifunctional crosslinker based on the amine-reactive N-hydroxysuccinimide (NHS) ester group.
Highlights:
- Can increase crosslinking efficiency compared to DSS in some applications
- Reactive groups: NHS ester (homobifunctional)
- Reactive toward: amino groups
Specifications
Specifications
| Chemical Reactivity | Amine-Amine |
| Cleavable | No |
| Molecular Weight (g/mol) | 326.26 |
| PEGylated | No |
| Spacer Arm Length | 7.7 Å |
| Content And Storage | Upon receipt store desiccated at 4°C. |
| Cell Permeability | Yes |
| Shipping Condition | Ambient |
| Product Line | Pierce |
| Labeling Method | Chemical Labeling |
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Frequently Asked Questions (FAQs)
DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.
NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information
For Research Use Only. Not for use in diagnostic procedures.
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