missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Immunohistochemistry Kits and Reagents
  5. 10321754

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Product Code. 10321754 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1 L
Unit Size:
1 set
Product Code. Quantity unitSize
10321754 1 L 1 set
1 options
This item is not returnable. View return policy

Product Code. 10321754

Brand: Thermo Scientific™ 36000

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Mouse Monoclonal Antibody

Thermo Scientific™ Pierce Peroxidase IHC Detection Kit bundles the Pierce Metal Enhanced DAB Substrate Kit with all necessary components to stain, counterstain and preserve experimental results with frozen or paraffin tissue sections.

The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The Thermo Scientific Pierce Metal Enhanced DAB Substrate Kit optimizes this intensifying chemistry, producing an exceptionally sensitive colorimetric detection system for Western blot, dot-blot and immunohistochemistry applications. The near-black signal (precipitate) is easy to photograph.

Highlights:

  • Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB

  • Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent

  • Six-hour stability – working solution is stable for more than six hours at room temperature! Other DAB substrates must be used immediately

  • Convenient – eliminates the need to procure numerous reagents from various sources

Includes:

DAB/Metal Concentrate (10X), 25mL; Universal Blocker Blocking Buffer in TBS, 250mL; Peroxidase Suppressor, 2 × 100mL; Stable Peroxide Buffer, 250mL; BupH Tris Buffered Saline, 4 packs; Surfact-Amps 20 (10% Tween-20), 10mL; Harris Modified Hematoxylin, 100mL; Mounting Medium, 60mL

TRUSTED_SUSTAINABILITY

Specifications

Detection Method Chromogenic, Colorimetric
Quantity 1 L
Content And Storage
  • DAB/Metal Concentrate (10X), 25 mL, store at -20°C (this solution will not freeze at -20°C)
  • Universal Blocker Blocking Buffer in TBS, 250 mL, store at 4°C
  • Peroxidase Suppressor, 2 × 100 mL, store at 4°C
  • Stable Peroxide Buffer, 250 mL, store at 4°C
  • BupH Tris Buffered Saline, 4 packs (each pack makes 500 mL), store at room temperature
  • Surfact-Amps 2 (1% Tween-2 Detergent), 10 mL, store at 4°C
  • Harris Modified Hematoxylin (without acetic acid, mercury-free), 100 mL, store at room temperature
  • Mounting Medium, 120 mL, store at room temperature
Substrate DAB (Enhanced)
Substrate Type HRP (Horseradish Peroxidase) Substrate
Form Liquid
Product Line Pierce
For Use With (Application) Immunodetection, IHC (Immunohistochemistry)
Product Type Peroxidase IHC Detection Kit

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.