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Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase

Product Code. 10414224 Shop All Thermo Scientific Products
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Quantity:
1 mg
5 x 1mg
Unit Size:
1mg
Pack of 5
Product Code. Quantity unitSize
10414224 1 mg 1mg
11849150 5 x 1mg Pack of 5
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Product Code. 10414224

Brand: Thermo Scientific™ 31487

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Description

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates.

Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate. Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines. Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody. After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine. The conjugate is purified by desalting or by dialysis. If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No. 44920) to separate unreacted enzyme from the conjugate.

Highlights:

  • Reacts with readily accessible primary amines to form a covalent secondary amine bond
  • Can be stored for at least 12 months at -20°C
  • Consistent conjugation yields reliable conjugates
  • Enzyme activity is 120-200 units/mg
TRUSTED_SUSTAINABILITY

Spezifikation

Chemical Reactivity Amine
Quantity 1 mg
Reactive Moiety Aldehyde
Solubility Water
Content And Storage Note: EZ-Link Plus Activated Peroxidase contains 1-3 moles of aldehyde per mole of peroxidase.

Storage: Upon receipt store product protected from moisture at -20°C. Product is shipped with an ice pack.
Form Solid
Product Line EZ-Link
Label Type Enzyme Labeled
Label or Dye HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.

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