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Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Aqueous

Product Code. 10414195 Shop All Thermo Scientific Products
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10414195 100 mL 1 set
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Product Code. 10414195 Supplier Thermo Scientific™ Supplier No. 23280
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Description

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This aqueous-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation includes sorbitol, which provides sensitivity enhancement. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects. This kit provides for maximum sensitivity with non-lipid samples.

Features of the Quantitative Peroxide Assay Kit:

  • Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
  • Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself

Applications:

  • General hydrogen peroxide (H2O2) measurement in protein samples
  • Quantitation of peroxides in detergents
  • Assessment of the effects of peroxides on cellular activity
  • Determination of protein glycation; Measurement of peroxide accumulation in lipids

In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Related Products

Pierce™ Quantitative Peroxide Assay Kit (Lipid) (Cat. No. 23285)

Pierce™ Peroxide Assay Reagent A (Cat. No. 23281)

Pierce™ Peroxide Assay Reagent B (Cat. No. 23282)

Pierce™ Peroxide Assay Reagent C (Cat. No. 23283)

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples
• Reagent A, 1 mL
• Reagent B, 2 x 50 mL

Store at 4°C.
Description Pierce Quantitative Peroxide Assay Kit (Aqueous)
Assay Quantitative Peroxide Assay
Detection Method Absorbance
Product Line Pierce
Product Type Quantitative Peroxide Assay
Specificity Not Target-Specific
Sufficient For 100 Tube Assays or 500 Microplate Assays
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 100 mL
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Safety and Handling

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Product Identifier
  • Quantitative Peroxide Assay Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: methanol

For Research Use Only. Not for use in diagnostic procedures.

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