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pJTI™ R4 Exp CMV EmGFP pA Vector

Product Code. 11681869
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Quantity:
100 μg
Unit Size:
100µg
This item is not returnable. View return policy

Product Code. 11681869

Brand: Invitrogen A14146

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This item is not returnable. View return policy

Description

pJTI™ R4 Exp CMV EmGFP pA Vector

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.

Ensure the Success of Your Jump-In™ Retargeting Reactions
Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:

Transfection efficiency

  • Cell confluency
  • Antibiotic selection conditions
  • Quality and concentration of DNA
  • Retargeting vector to integrase vector ratio

    We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Constitutive or Inducible System Constitutive
    Delivery Type Transfection
    Format Liquid
    Promoter CMV
    Product Type Mammalian Expression Vector
    Selection Agent (Eukaryotic) None
    Content And Storage Immediately upon receipt, store vector at -20°C
    Shipping Condition Dry Ice
    Protein Tag Untagged
    Cloning Method Gateway
    Quantity 100 μg
    Vector Jump-In Vectors
    Product Line Jump-In™
    For Use With (Application) Stable Cell Line Development, Targeted Integration
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    For Research Use Only. Not for use in diagnostic procedures.

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