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Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Description
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.
Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.
Features
- Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
- Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
- Same performance as Platinum Taq DNA Polymerase manufactured using conventional process
Applications
- Amplification of DNA from complex genomic and viral templates
- Hot start PCR
- RT-PCR
Specifications
Specifications
| Concentration | 5 U/μL |
| Content And Storage | • Platinum Taq DNA Polymerase, DNA-free, 100 μL • 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL • 50 mM MgCl2, DNA-free (1 mL) Store at -20°C in a non-frost-free freezer. |
| Format | Tube |
| GC-Rich PCR Performance | Low |
| Polymerase | Platinum Taq DNA Polymerase |
| Reaction Speed | Standard |
| Product Type | Taq DNA Polymerase |
| Quantity | 500 Units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Hot-start PCR |
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Frequently Asked Questions (FAQs)
Platinum Taq DNA Polymerase, DNA-free is available in 5.0 U/µL enzyme concentration.
Yes. Due to stable antibody-mediated hot-start technology, Platinum Taq DNA Polymerase, DNA-free is highly stable. The PCR reactions can be assembled at room temperature without any loss of amplification specificity.
Amplification signal in “no-template controls” (NTC), also known as false-positive signal, or background amplification in “reagent-only control”, may arise from contaminating DNA that entered control reactions from the environment, from the researcher, or was introduced into the PCR via contaminated PCR reagents or consumables. To avoid contaminating DNA in your experiments, it is important to follow these recommendations:
- Be cautious to maintain a DNA-free environment during handling and opening the vials with DNA polymerase, PCR buffer, and MgCl2
- Ensure that all components of the PCR, like dNTPs, primers, probes, or water are free of contaminating DNA that can give false positive PCR results
- Avoid opening the tubes containing DNA-free reagents multiple times to minimize risk of DNA contamination
- Work in a UV-irradiated workstation
- Use certified DNA-free pipette tips and PCR consumables
PCR reactions with Platinum Taq DNA Polymerase, DNA-free can be run using the same cycling protocol as that for standard Taq.
Yes, Platinum Taq DNA Polymerase, DNA-free can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.
For Research Use Only. Not for use in diagnostic procedures.
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