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  5. Invitrogen™ Rat IL-4 ELISA (For Lysates) Kit

Invitrogen™ Rat IL-4 ELISA (For Lysates) Kit

Product Code. 17155043
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96 Tests
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17155043 96 Tests Pack of 96
17135869 5 x 96 Tests Each
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Product Code. 17155043 Supplier Invitrogen™ Supplier No. ERA30RB

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Description

ELISA

Rat IL-4 quantitates rat IL-4 in cell lysate, tissue homogenate. The assay will exclusively recognize both natural and recombinant rat IL-4.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number B cell growth factor 1; B
Assay Range 1.5 to 160 pg/mL
Assay Sensitivity 1.5 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates, Tissue Homogenates
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 287287
Gene Symbol Il4
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 50 μL, Tissue Homogenate 50 μL
Storage Requirements 2°C to 8°C
Target IL-4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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