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Invitrogen™ ReadyLabel™ Antibody Labeling Kits
Beskrivning
ReadyLabel Antibody Labeling Kits enable fast, convenient labeling of off-the-shelf antibodies and cell culture supernatants without prior purification of the antibody solution. Each kit contains everything needed to perform 5 labeling reactions and will produce purified antibody conjugates, without the purification step.
Each ReadyLabel Antibody Labeling Kit, 100 μg contains everything needed to perform 5 labeling reactions and will produce purified antibody conjugates, without the purification step. The kit can be used to label antibodies in solutions containing BSA or other stabilizing proteins and antibodies in a primary amine-containing buffer such as Tris or glycine, or even cell culture supernatants, without prior purification of the antibody-containing solution. A reactive dye moiety efficiently binds to primary amines of antibodies to form stable, covalently bound antibody-dye conjugates.
Alexa Fluor dyes produce antibody conjugates that are typically brighter with better photostability and lower pH sensitivity relative to traditional dyes (e.g., FITC). Unlike other dyes, the fluorescence of Alexa Fluor conjugates are insensitive to pH between 4 and 10. Alexa Fluor-labeled antibodies can be used for applications including microscopy, flow cytometry, western blotting, ELISA, and indirect FISH.
ReadyLabel Antibody Labeling Kits offer the following benefits:
- Convenience—Labeling takes one hour, with less than 20 minutes of hands-on time
- Versatility—Labelling can be performed on IgG antibodies, regardless of species, isotype, or storage buffer
- Customization—ReadyLabel Flex Antibody Labeling Kits are designed for use with a wide range of amine-reactive labels including Alexa Fluor dyes
Specifikationer
Specifikationer
| For Use With (Application) | Antibody Production and Labeling |
| Product Type | Antibody Labeling Kit |
| Quantity | 5 x 100 μg of IgG |
| For Use With (Equipment) | Flow Cytometer, Fluorescence Microscope |
| Product Line | ReadyLabel |
| Chemical Reactivity | Amine |
| Solubility | DMSO (Dimethylsulfoxide) |
| Label or Dye | Alexa Fluor 647 |
| Color | Far-red |
Frequently Asked Questions (FAQs)
ReadyLabel Columns bind poorly to rat and bovine antibodies, and are therefore, not recommended to use with the ReadyLabel Antibody Labeling Kits (Cat. Nos. R10701, R10702, R10709, R10710, R10711, R10712, R10713, R10714).
For Research Use Only.
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