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STEMCELL Technologies RSeT™ Feeder-Free Medium

Product Code. 16467933
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500 mL Kit
Unit Size:
500mL
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16467933 500 mL Kit 500mL
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Product Code. 16467933 Supplier STEMCELL Technologies Supplier No. 05975

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Description

Serum-free medium for naïve-like human pluripotent stem cells

  • Revert primed human embryonic (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintain cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells
  • Developed under license from the Weizmann Institute of Science, this serum-free cell culture medium produces robust cultures with features of a naïve-like state, such as tightly packed, domed colonies with refractive edges
  • Human ES/iPS cells cultured in RSeT Feeder-Free Medium show increased expression of key transcripts associated with the naïve-like state, such as KLF17, KLF2, KLF4, and TFCP2L1, and can either be differentiated or converted back to a primed state by culture in mTeSR1 and then differentiated
  • Compatible products that can be used for differentiation include STEMdiff Definitive Endoderm Kit (Catalog #05110), STEMdiff SMADi Neural Induction Kit (Catalog #08581), and STEMdiff Mesoderm Induction Medium (Catalog #05220)

Specifications

Cell Type Pluripotent Stem Cells
For Use With (Application) Cell Culture, Expansion, Maintenance
Quantity 500 mL Kit
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