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Ambion™ Silencer™ Pre-Designed siRNA
Beschreibung
Silencer Predesigned siRNAs are high-quality, synthetic small interfering RNAs (siRNAs) designed for gene silencing applications in mammalian cells. This cost-effective RNA interference tool delivers reliable gene knockdown performance while maintaining economic accessibility for research applications. Our siRNA products are meticulously crafted to help ensure high efficiency and specificity, enabling researchers to achieve reliable and reproducible gene knockdown results. Silencer Predesigned siRNAs are unmodified synthetic RNA duplexes with a 19-mer duplex and two-nucleotide 3' overhangs.
Key features include:
- High silencing efficiency—demonstrates good knockdown performance with optimized target recognition for effective gene expression suppression
- Enhanced specificity—algorithm designed for precise gene targeting to minimize off-target effects and help ensure selective gene silencing
- Guaranteed performance—two out of three predesigned siRNAs will result in >70% gene knockdown. Validated designs available for many common genes.
- Cost-effective solution—exceptional value for research applications without compromising performance quality
Streamlined workflow for rapid results
Utilizing our gene knockdown technology is straightforward and efficient. Simply order siRNAs for the desired gene targets, deliver to the cells, and detect knockdown efficiency and validate the resulting phenotype. Take advantage of our validated designs for better confidence in your experiments. This streamlined process allows for rapid experimental progress and accelerated discovery in gene function studies.
Spezifikation
Spezifikation
| Content And Storage | Silencer™ Select siRNAs are the result of recent improvements in siRNA design combined with novel chemical modifications yielding unrivaled knockdown efficiency and specificity. Our best performing siRNA for cell culture work. Silencer™ siRNAs are unmodified 21 nucleotide siRNAs that have been designed with an older, yet rigorously tested design algorithm. A lower cost alternative to Silencer™ Select siRNAs, but without some of the performance enhancements. |
| Description | Silencer Pre-Designed siRNA STD, 20nmol, Incorporate the Latest Improvements in siRNA Design, Off-Target Effect Prediction Algorithms, and Chemistry, Guaranteed-to-Silence Based on their Proven Design |
| Format | 96-well Plate, 384-well Plate |
| RNAi Type | siRNA |
| Sample Type | mRNA |
| Final Product Type | Silencer Pre-Designed siRNA STD |
| Label or Dye | Unlabeled |
| Product Line | Silencer, Ambion |
| Product Type | Silencer™ Pre-Designed Standard siRNA |
| Purity | Standard |
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Frequently Asked Questions (FAQs)
For any RNAi duplex (miRNA or siRNA) there is a possibility that adding a modification will affect how the antisense strand interacts with RISC and aligns with its mRNA target, thus impacting functionality. For that reason, we recommend that you label the 5' end of the sense strand for siRNA (or passenger strand for miRNA). Since the 5' end of the antisense (guide strand) is what gets recognized by RISC, it is best to add the fluorescent modification to the end of the duplex on the strand that does not interact with RISC. You can also test a labeled vs. non-labeled RNAi duplex to determine if the modification is affecting the siRNA or miRNA functionality.
Please contact Technical Support at techsupport@thermofisher.com to request the GC content for one of our predesigned Silencer siRNA or Silencer Select siRNA sequences.
For Research Use Only. Not for use in diagnostic procedures.
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