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IBA Solutions For Life Science StarGate™ pPSG-IBA Acceptor Vectors for E. coli Expression
Achieves high-level expression in E. coli by the T7 promoter by providing specified genetic surroundings for optimal expression and purification of non-toxic proteins.
Brand: IBA Solutions For Life Science 5-4203-001
Additional Details : Poids : 0.00010kg
Description
pPSG-IGA E. coli expression vectors:
pPSG-IBA vectors use the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest. As the T7 promoter is stronger than the tet promoter, pPSG-IBA vectors are recommended in cases where expression with the tet promoter does not lead to significant yields of the recombinant protein.
- High-level expression in E. coli by bacteriophage T7 promoter
- High-level transcription by T7 RNA polymerase in BL21 strains
- High-level expression of non-toxic proteins
- Induction by IPTG
- Suitable for in vitro transcription/translation
StarGate™ System
IBA developed StarGate to provide a simple, fast and highly efficient cloning process for genes of interest (GOI) for use in functional expression, purification, detection, immobilization or separation of recombinant proteins:
- Minimal extra modification of the gene due to short combinatorial sites
- Inherent high-level cloning efficiency due to a directed reaction (no equilibrium)
- GOIs are cloned to obtain a Donor Vector; 2 or more genes can be combined using Fusion Cloning
- Genes are then transferred into Acceptor Vectors that provide optimal genetic surroundings
- The final expression vector, the Destination Vector, is then placed into the respective host
Specifications
| 250 ng/μL | |
| Clear | |
| StarGate™ system, E. coli BL21(DE3) | |
| E. coli host with T7 RNA polymerase gene (e.g. BL21(DE3) ) | |
| 8 | |
| Acceptor Vector for E. coli expression | |
| Total length: 3469 | |
| 20μL TE buffer: 10mM Tris-HCl, 1mM EDTA per 5μg |
| Cloning genes of interest for use in functional expression, purification, detection, immobilization or separation of recombinant proteins | |
| Liquid | |
| pPSG-IBA 3 | |
| Ampicillin Resistance cassette | |
| T7 | |
| 5 μg | |
| 2-8°C for frequent usage, -20°C for long-term storage | |
| Strep-tag™ for C-terminal |