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  6. IBA Lifesciences Strep-Tactin™ TACS Agarose Column

IBA Lifesciences Strep-Tactin™ TACS Agarose Column

Product Code. 17287590 Shop All IBA Lifesciences Products
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Capacity (Metric):
0.3 mL
1 mL
Unit Size:
Each
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17287590 0.3 mL Each
17227750 1 mL Each
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Product Code. 17287590 Supplier IBA Lifesciences Supplier No. 66310300
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Description

Gravity flow column suitable for traceless affinity cell and exosome isolation

  • Strep-Tactin™ TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
  • A special crosslinking permits the use for cell isolation and depletion approaches.
  • They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS™/Nano-TACS™).
  • In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
  • After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
  • This way the cells are eluted ready for further downstream applications.
  • The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes).

  • Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding).
  • This makes Strep-Tactin™ TACS Agarose columns also suitable for negative cell selection or depletion approaches.

Specifications

Capacity (Metric) 0.3 mL
Formulation Pre-packed in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide
Support Type Cross-linked agarose
For Use With (Application) Affinity Chromatographic Cell or Exosome Isolation

For Research Use Only.

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