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Applied Biosystems™ TaqMan™ miRNA ABC Purification Kit - Human Panel B
TaqMan™ miRNA ABC Purification Kit - Human Panel B
Brand: Applied Biosystems™ 4473088
Additional Details : Weight : 0.00450kg
Description
Use the TaqMan™ miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.Simple and Fast miRNA Purification
The TaqMan™ miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes (see workflow, Figure 1).
Human Panel A and Human Panel B
The TaqMan™ miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B. The beads in each kit are superparamagnetic Dynabeads™ covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan™ Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads. Complete lists of miRNAs isolated by the panels can be found in the TaqMan™ miRNA ABC Purification Kit protocol (below).
miRNA Analysis
The miRNAs isolated using the TaqMan™ miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan™ microRNA assays or the TaqMan™ Array Human microRNA A and B cards. Figure 2 gives examples of the data that can be obtained from miRNA isolated from various sample types using the TaqMan™ miRNA ABC Purification Kit.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Specifications
| 2°C to 8°C | |
| Lysis Buffer, ABC Buffer, Wash Buffer 1 and 2, Elution Buffer | |
| Human | |
| micro RNA | |
| Lysis buffer, ABC buffer, wash buffer 1, wash buffer 2, elution buffer, 1.5mL loBind tubes | |
| TaqMan™ Human Panel B miRNA ABC Purification Kit | |
| 2°C to 8°C |
| TaqMan™ Human Panel B miRNA ABC Purification Kit, 40 preps, Liquid, Magnetic Bead Isolation Technology, Human Species, Dynabeads™ System, Blood, Cells, FFPE and Fixed Samples, Liquid Samples Sample Type, micro RNA Final Product, 75 min Purification Time, 2 to 8°C Storage | |
| Magnetic Bead | |
| Blood, Cells, Whole Blood (Specific), FFPE and Fixed Samples, Liquid Samples (e.g. Serum), FFPE and Fixed Samples, Liquid Samples (e.g. Serum), Plasma, Tissue, Serum, Tissue, Tissue (FFPE) | |
| Real-time quantitative PCR (qPCR), microRNA analysis, RNA extraction, miRNA and non-coding RNA analysis, DNA and RNA purification and analysis, RNAi, epigenetics and non-coding RNA research, miRNA and non-coding RNA analysis, miRNA isolation | |
| 1 Each | |
| 40 Preps | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.