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Applied Biosystems™ TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited

Product Code. 15621812 Shop All Applied Biosystems Products
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Product Code. 15621812 Supplier Applied Biosystems™ Supplier No. 4485715

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Enables relative gene expression quantification in cDNA samples when used with other gene expression assays

  • The Applied Biosystems™ TaqMan™ RNase P Assay with ABY™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human RNase P.
  • This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of an ABY™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers.
  • The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress RNase P.
  • Eliminate assay design time using pre-designed primers and pre-designed ABY™ dye-labeled TaqMan™ probe
  • Minimize experimental optimization time
  • Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
  • Formulated at a primer-limited concentration for use in multiplex reactions
  • Save time in assay design, formulation, and testing by using the TaqMan™ RNase P Assay as your control when quantifying gene expression.
  • This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
  • In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high RNase P expressors. The ABY™ dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.
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Specifications

Accession Number NR_002312.1
Amplicon Size 87
Content And Storage Primer-Probe Set(s)
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Gene Symbol RNase P (RPPH1)
Internal Probe Modification QSY™ Non-Fluorescent Quencher (3')
Label or Dye ABY
Product Line TaqMan
Product Type Real Time PCR Reagents
Quantity 250 reactions
Species Human
Target Ribonuclease P RNA component H1
For Use With (Application) Real Time PCR (qPCR)
No. of Reactions 250 reactions
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For Research Use Only. Not for use in diagnostic procedures.

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