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Applied Biosystems™ TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited
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- The Applied Biosystems™ TaqMan™ RNase P Assay with ABY™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human RNase P.
- This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of an ABY™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers.
- The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress RNase P.
- Eliminate assay design time using pre-designed primers and pre-designed ABY™ dye-labeled TaqMan™ probe
- Minimize experimental optimization time
- Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
- Formulated at a primer-limited concentration for use in multiplex reactions
- Save time in assay design, formulation, and testing by using the TaqMan™ RNase P Assay as your control when quantifying gene expression.
- This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
- In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high RNase P expressors. The ABY™ dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.
Specifications
Specifications
| Accession Number | NR_002312.1 |
| Amplicon Size | 87 |
| Content And Storage | Primer-Probe Set(s) |
| Detection Method | Primer-probe |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Gene Symbol | RNase P (RPPH1) |
| Internal Probe Modification | QSY™ Non-Fluorescent Quencher (3') |
| Label or Dye | ABY |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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