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Description
Thermo Scientific GeneJET FFPE DNA Purification Kit is designed for fast and convenient purification of DNA from various amounts of archived FFPE tissue. Up to 8 sections (10 μm thickness) of FFPE sample from various tissues can be used for genomic DNA extraction without overnight incubation. Elimination of toxic reagents commonly used for deparaffinization (i.e., xylene) allows a safe DNA purification procedure with high DNA yields and excellent quality. Each preparation recovers up to 8 μg of genomic DNA that can be eluted in volumes from 20 μL allowing for concentrated samples. Due to high decrosslinking efficiency high quality purified DNA shows reliable performance in sensitive downstream applications such as qPCR, PCR (amplicons up to 1 kb) or NGS library preparation.
Highlights
• High quality DNA—suitable for PCR and qPCR
• Fast—procedure can be completed within 2 hours
• Safe—non toxic solvents
• Efficient—yields up to 8 μg of purified DNA
Applications
• PCR
• qPCR
• NGS library preparation
Includes
• Proteinase K Solution
• RNase A Solution
• Digestion Buffer
• Binding Buffer
• Wash Buffer I (conc.)
• Wash Buffer II (conc.)
• Elution Buffer
• GeneJET DNA Purification Columns & Collection Tubes
• Collection Tubes
• Detailed Protocol
Order Info
Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.
Especificaciones
Especificaciones
| Column Type | Spin Column |
| Format | Spin Column |
| Isolation Technology | Spin Column |
| Sample Type | FFPE, Fixed Samples |
| For Use With (Application) | Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| No. of Reactions | 50 Preps |
| Product Line | GeneJET |
| Product Type | FFPE DNA Purification Kit |
| Quantity | 50 Preps |
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Seguridad y manipulación
- GeneJET FFPE DNA Purification Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: Proteinase, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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