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Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution
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Thermo Scientific Triton X-100 Surfact-Amps Detergent Solution is a highly-purified Triton™ X-100 detergent stabilized as a 10% solution (w/v) in variable sizes packaged under nitrogen in 10 mL glass ampules or three sizes of non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.
Features of Triton X-100 Surfact-Amps Detergent Solution:
- Triton X-100—a nonionic detergent for use in various protein methods
- Accurate—precise 10% detergent solution in ultrapure water
- Easy-to-use—solution is simple to dispense and dilute for use
- Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
- Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles
Specifications for Triton X-100 Surfact-Amps Detergent Solution:
- Visual: Clear to slightly hazy, colorless solution, free of particulate matter.
- Concentration: 10.0±1.0%
- Oxidants: ≤1.0μeq/mL
- Carbonyls: ≤1.0μeq/mL
- Suspended Solids: Residue present must not exceed Residue Reference.
Properties of Triton X-100:
- Molecular Weight: 647 g
- Detergent Class: Nonionic polyoxyethylene surfactant
- Aggregation Number: 140
- Micelle Molecular Weight: 90,000 g
- Critical Micelle Concentration (CMC): 0.24 mM (0.0155% w/v)
- Cloud Point: 64°C
- Dialyzable: No
Triton X-100 is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins.
Related Products
- Surfact-Amps™ Detergent Sampler (Cat. No. 28340)
Specifications
Specifications
| Content And Storage | Store between 20°C and 25°C |
| Form | Liquid |
| Product Line | Surfact-Amps, Triton |
| Reagent Type | Detergent Solution |
| Quantity | 250 mL |
Frequently Asked Questions (FAQs)
One thing you should do is to permeabilize well, by including 0.1% to 0.2% Triton X-100 in all washes and incubations. Another recommendation is to extend the labeling times to overnight at 4oC for both primary antibody and secondary antibody. Finally, label the section free-floating, rather than adhered to a slide, to gain penetration for the antibody from both sides.
After opening, we recommend storing the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. Frequent opening should be avoided because Triton X-100 is susceptible to autoxidation upon exposure to air.
Triton X-100 Surfact-Amps Detergent Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (20-25 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
Safety and Handling
- Triton X-100 Surfact-Amps Detergent Solution
- Danger
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- H318-Causes serious eye damage.
- H412-Harmful to aquatic life with long lasting effects.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy
For Research Use Only. Not for use in diagnostic procedures.
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