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Thermo Scientific™ Verso cDNA Synthesis Kit
Description
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.
Features of the Verso cDNA Synthesis Kit include:
• Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR
Applications
• 2-step RT-(q)PCR
• Single stranded cDNA synthesis
Specifications
Specifications
| Content And Storage | • RT Enzyme mix with RNase Inhibitor |
| Format | Kit |
| Reaction Speed | 30 min. |
| Technique | Reverse Transcription |
| Optimal Reaction Temperature | 42°C |
| Quantity | 100 reactions |
| Reverse Transcriptase | Verso |
| Ribonuclease H Activity | Yes |
| Shipping Condition | Wet or Dry Ice |
| For Use With (Application) | Real Time PCR (qPCR), RT-PCR |
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Frequently Asked Questions (FAQs)
The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.
No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.
Error rate is approximately 1 error per 15-27x 10^3 bases.
The Verso can synthesize long cDNA strands, up to 11 kb, at a temperature range of 42 degrees C to 57 degrees C. For improved performance, we recommend SuperScript IV Reverse Transcriptase.
The Verso cDNA synthesis Kit enables robust transcription of RNA to create a complete cDNA pool. The Verso system allows robust and sensitive reverse transcription through the combination of a high-affinity RT enzyme, a trusted RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.
For Research Use Only. Not for use in diagnostic procedures.
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