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Invitrogen™ ApoA1 Monoclonal Antibody (6001)
Mouse Monoclonal Antibody
Brand: Invitrogen™ MIA1401
This item is not returnable.
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Description
By sandwich ELISA, MIA1401 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MIA1401 is used for detection.MIA1401 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1401, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MIA1401 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1401 may not be as successful for detecting endogenous Apo A-1 by Western blot. MIA1401 was formerly sold as a Seradyn product.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
Specifications
| ApoA1 | |
| Monoclonal | |
| 5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.4 | |
| P02647, P04639, Q00623 | |
| APOA1 | |
| Purified human plasma Apo A-1. | |
| 1 mg | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG2a |
| ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmune Assays (RIA), Western Blot | |
| 6001 | |
| Unconjugated | |
| APOA1 | |
| 1-Sep; 2-Sep; Alp-1; Apo a; APO A1; apo A-1; APO I; apo(a); APOA1; Apoa-1; ApoAI; Apo-AI; apoA-I; Apolipoprotein; Apolipoprotein A1; apolipoprotein A-1; Apolipoprotein AI; apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Brp-14; Ltw-1; Lvtw-1; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Sep-1; Sep2; Sep-2; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7 | |
| Mouse | |
| Protein A | |
| RUO | |
| 11806, 25081, 335 | |
| Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C | |
| Liquid |
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